Day 16 : Bablassss; PCR+Gel Electrophoresis_Part 4

Today, i just arrived from Kaohsiung at 3.45am. TMS was still very quiet at that time. After waited for couple hours, i took MRT and headed to NTU dormitory. Because it was too early, so, nobody's in and out the dorm. Then i decided to go to NTUST dorm (*my second headquarters^^). Took a shower and went to the bus stop, as always. After i sat at the bus, i felt very sleepy and...finally fell asleep very tightly. I don't even noticed that i've already arrived at Academia Sinica. Suddenly, i woke up and swept my eyes to the surroundings. Nobody was there. And...i saw my watch. It was 10.05. HECK!. Usually, the bus will arrive there between 9.30-9.40 and soon left after all of the passengers are get off from the bus. But, hahahaaaa...it was already 10.05 and the bus still there. I didn't even see the driver. OMG...i guess because of me, today's bus schedule was messed up. Hehehe...sumimasen deshita^^. But, that wasn't the main problem. I was L.A.T.E for my 9.30's lab meeting. *i'm so dead*

Thank God Dr. Tang and everybody were just very nice. They can understand the "ridiculous situation" i've faced. They even laughed at me (*'til they got stomach cramps, i guessed) after i told them my story.

Today, i have to do PCR and Gel Electrophoresis again. I still couldn't get my PCR products last Friday, so..TRY AGAIN^^. And...i managed to get those PCR products. Alhamdulillah^^. Well, next is waiting for PCR products sequencing:).

Btw, today, Ching-Hung a.k.a John, my genius lab mate, was acting a little bit weird. Last week, he didn't show up at lab. Then, i just knew from Dr. Chiou (*my other lab mate) that he was travelled around Taiwan for the whole week with...his girlfriend ;p. Hohohoooo...

Usually, Ching-Hung always brings his lunchbox to the "dining room for all" outside the lab. FYI, that "dining room" is my cubicle here^^. He always takes his lunch box and newspaper(s) along with him and chit-chat with me. Today, because i was a little bit "chased by deadlines" about a lot of things, i don't really wanted to talk to him. So, when he just blurted "Bunga...", i replied "Nice new haircut, Ching-Hung". And then, he blushed and...left speechlessly. Hahahaaaa....*mulai kurang ajar mode on ;p*

Day 15 : PCR + Gel Electrophoresis_Part 3 ; Sayonara Sleepy Head ;p

Whew! Yesterday's PCR and Gel Electrophoresis results weren't good at all. There was no PCR products can be detected on the gel. That's why today, i'm doing it again^^.

While waiting for my running PCR, i went out for my summer course class. Something amazing happened. I finally graduated as a sleepy head ^^V. I listened to the whole lesson carefully, write down some important notes and recorded the course materials on my mp3. Perfect^^.

Btw, there was a phrase from Louis Pasteur that quoted by Dr. Chang in the class :

“Chances favors the prepared mind”

"Only a deep thinker, the one that work hard and smart, able to grasp the opportunity", said Dr. Chang.

See this picture below and go here for some details.

Besides that, he also said :

"You have to know the scientific history, so you won't make the same mistakes like the former scientists did".

Interesting, right? ^^

Day 14 : PCR + Gel Electrophoresis_Part 2

Because my last week results weren't so good, today i have to run PCR and Gel electrophoresis again. But of course, with the different colonies. I've discussed with Dr. Tang and Mia about too high similarity in last week's results. High similarity in the isolates indicates lack of biodiversity. And don't dare to think even further about getting some novel microbes if your results are just like mine. So, we decided to inoculate white colored colonies and reddish colored colonies. Before, i only inoculated pink colored colonies. Well, maybe the white and the reddish ones are the novel microbes, or at least, they have low similarity. So, GANBARIMASU^^!

*Note :

1. Isolate 1- 5 are reddish colored colonies; isolate 6-10 are white colored colonies.
2. Always remember to make the gel agarose first after loading the samples to PCR.